XTT Cell Proliferation Assay Kit (CV0005)
- SKU:
- CV0005
- Product Type:
- Assay
- Research Area:
- Cell Health
Description
XTT Cell Proliferation Assay Kit
XTT Cell Proliferation Assay Kit is an optimized, accurate and sensitive colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT (sodium 2,3,-bis(2methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium) is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells. This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample.
The formazan dye formed in the assay is soluble in aqueous solution and quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as PMS (N-Methylphenazonium methyl sulphate), can significantly improve the efficiency of XTT reduction in cells.
Features & Benefits
| Overview | Description |
| Size: | 500/1000 Assays |
| Accurate: | Dye absorbance is proportional to the number of cells in each well. |
| Sensitive: | Assayed even in low cell concentrations. |
| Fast protocol: | Minimal handling steps & results within 2-5 hours, no solubilization step required. |
| Complete solution: | Includes all reagents needed for cell washing procedures. |
| Safe: | Non-radioactive. |
| Optimized: | Developed for high throughput assays (no washing or other steps that can cause cell loss and variability). |
| Applications: |
|
| Storage & Shipping: |
|
Buffers & Reagents:
| Size | |
| 500 Assays: |
|
| 1000 Assays: |
|
Related Products
MTT Cell Proliferation and Cytotoxicity Assay Kit
