The XPC Polyclonal Antibody (CAB8354) is a valuable tool for researchers studying the XPC protein, which plays a key role in DNA repair mechanisms. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blot applications. By binding to the XPC protein, it allows for the detection and analysis of XPC expression in a variety of cell types.XPC is essential for the recognition and repair of damaged DNA, making it crucial for maintaining genomic stability and preventing mutations that can lead to cancer and other diseases.
Research on the function and regulation of XPC is important for understanding the mechanisms of DNA repair and how disruptions can contribute to genetic disorders and cancer development.By utilizing the XPC Polyclonal Antibody, researchers can further investigate the role of XPC in DNA repair processes, tumor suppression, and genetic stability. This antibody is a valuable resource for studies in molecular biology, oncology, and genetic research, providing insights into the intricate mechanisms that safeguard the integrity of the genome.
Product Name:
XPC Rabbit Polyclonal Antibody
SKU:
CAB8354
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 700-940 of human XPC (NP_004619.3).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
XP3; RAD4; XPCC; p125; XPC
Positive Sample:
LO2,HeLa,293T,HT-1080,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
106kDa
Observed MW:
130kDa
The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7508
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using XPC Rabbit pAb (CAB8354) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 5s.
