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XPA Colorimetric Cell-Based ELISA Kit

SKU:
CBCAB00208
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Mouse
Detection Method:
Colorimetric
€599
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Description

system_update_altDatasheet

XPA Colorimetric Cell-Based ELISA Kit

The XPA Colorimetric Cell-Based ELISA Kit is a cutting-edge tool for researchers looking to accurately measure XPA (Xeroderma Pigmentosum Group A) levels in cell lysates and tissue samples. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.XPA is a critical protein involved in DNA repair processes, particularly in the recognition and removal of DNA damage caused by UV radiation. Dysregulation of XPA has been linked to various diseases, including Xeroderma Pigmentosum and certain types of cancer.

By accurately measuring XPA levels, researchers can gain valuable insights into DNA repair mechanisms and disease progression, paving the way for potential therapeutic interventions.The XPA Colorimetric Cell-Based ELISA Kit is user-friendly, requiring minimal hands-on time and offering quick and easy data analysis. With its high performance and reliability, this kit is an indispensable tool for researchers studying DNA repair mechanisms, genetic disorders, and cancer biology.

Product Name:XPA Colorimetric Cell-Based ELISA
Product Code:CBCAB00208
ELISA Type:Cell-Based
Target:XPA
Reactivity:Human, Mouse
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The XPA Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect XPA protein expression profile in cells. The kit can be used for measuring the relative amounts of XPA in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on XPA.

Qualitative determination of XPA concentration is achieved by an indirect ELISA format. In essence, XPA is captured by XPA-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 7507, UniProt ID: P23025, OMIM: 278700/611153, Unigene: Hs.654364
Gene Symbol:XPA
Sub Type:None
UniProt Protein Function:XPA: Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. Interacts with GPN1 and RPA1. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2. Expressed in various cell lines and in skin fibroblasts. Belongs to the XPA family.
UniProt Protein Details:

Protein type:DNA repair, damage

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytoplasm; DNA replication factor A complex; Golgi apparatus; intercellular bridge; nucleoplasm; nucleus

Molecular Function:damaged DNA binding; protein binding; protein domain specific binding; protein homodimerization activity

Biological Process: base-excision repair; DNA repair; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; transcription-coupled nucleotide-excision repair

Disease: Xeroderma Pigmentosum, Complementation Group A

NCBI Summary:This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:P23025
NCBI GenInfo Identifier:139816
NCBI Gene ID:7507
NCBI Accession:P23025.1
UniProt Secondary Accession:P23025,Q5T1U9, Q6LCW7, Q6LD02,
UniProt Related Accession:P23025
Molecular Weight:31,368 Da
NCBI Full Name:DNA repair protein complementing XP-A cells
NCBI Synonym Full Names:XPA, DNA damage recognition and repair factor
NCBI Official Symbol:XPA  
NCBI Official Synonym Symbols:XP1; XPAC  
NCBI Protein Information:DNA repair protein complementing XP-A cells
UniProt Protein Name:DNA repair protein complementing XP-A cells
UniProt Synonym Protein Names:Xeroderma pigmentosum group A-complementing protein
Protein Family:DNA repair protein
UniProt Gene Name:XPA  
UniProt Entry Name:XPA_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-XPA Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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