XKR8 Antibody (PACO47106) is a high-quality monoclonal antibody specifically designed for research involving XKR8, a recently discovered protein involved in cellular transport and membrane integration processes. The antibody is derived from mouse monoclonal cells and is highly reactive with human, mouse, and rat samples, making it versatile for a wide range of research applications.XKR8 plays a crucial role in mediating phosphatidylserine translocation across cell membranes, a process that is essential for various cellular functions, including apoptosis and phagocytosis. Its involvement in these processes makes it a key player in immune response regulation and cancer progression.
This antibody is validated for use in immunofluorescence, immunoprecipitation, and flow cytometry applications, allowing for the detection and analysis of XKR8 in different cell types and tissues. Its specificity and sensitivity make it an ideal tool for researchers studying membrane biology, cellular transport, and signal transduction pathways.Overall, the XKR8 Antibody (PACO47106) is a valuable tool for researchers interested in understanding the role of XKR8 in cellular function and its potential implications in disease processes. Its high reactivity and reliability make it a trusted reagent for studies in cell biology, immunology, and cancer research.
Western Blot. Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate. All lanes: XKR8 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa..
IHC image of PACO47106 diluted at 1:1000 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO47106 diluted at 1:1000 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Promotes phosphatidylserine exposure on apoptotic cell surface, possibly by mediating phospholipid scrambling. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment. Has no effect on calcium-induced exposure of phosphatidylserine. Activated upon caspase cleavage, suggesting that it does not act prior the onset of apoptosis.
Synonyms:
XK-related protein 8 (hXkr8) [Cleaved into: XK-related protein 8, processed form], XKR8, XRG8
UniProt Protein Function:
XKR8: Belongs to the XK family.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 1p35.3Cellular Component: plasma membraneBiological Process: engulfment of apoptotic cell
