The XDH Polyclonal Antibody (CAB13052) is a valuable tool for researchers studying XDH, an enzyme involved in purine metabolism and oxidative stress regulation. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications such as Western blotting.XDH, also known as xanthine dehydrogenase, plays a crucial role in the conversion of hypoxanthine to xanthine and xanthine to uric acid, a process essential for maintaining proper purine levels in the body. Additionally, XDH is involved in the generation of reactive oxygen species and reactive nitrogen species, leading to its implications in oxidative stress and inflammatory responses.
This antibody binds specifically to the XDH protein, allowing for its detection and analysis in different cell types and tissues. Researchers in fields such as biochemistry, molecular biology, and pharmacology can benefit from using this antibody to further understand the function and regulation of XDH in various physiological and pathological conditions. By studying XDH, researchers can uncover potential therapeutic targets for diseases related to purine metabolism dysregulation and oxidative stress.
Product Name:
Xanthine Oxidase (XDH) Rabbit Polyclonal Antibody
SKU:
CAB13052
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 985-1084 of human Xanthine Oxidase (XDH) (NP_000370.2).
Sequence:
ETST NTVP NTSP TAAS VSA
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
XO; XOR; XAN1; Xanthine Oxidase (XDH)
Positive Sample:
LO2,Mouse liver,Rat lung,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Peroxisome, Secreted.
Calculated MW:
146kDa
Observed MW:
150kDa
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
Purification Method:
Affinity purification
Gene ID:
7498
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Xanthine Oxidase (XDH) antibody (CAB13052) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
