The XBP1 Polyclonal Antibody (CAB1731) is a valuable tool for researchers studying XBP1, a transcription factor involved in the unfolded protein response pathway. This antibody is produced by Assay Genie and is highly specific for human samples, making it ideal for experiments involving XBP1 in various cell types.XBP1 is essential for the cellular response to endoplasmic reticulum stress and is a key player in maintaining protein homeostasis. Dysregulation of XBP1 has been implicated in a variety of human diseases, including cancer, neurodegenerative disorders, and metabolic diseases.
Understanding the role of XBP1 in these conditions could lead to the development of novel therapeutic strategies.The XBP1 Polyclonal Antibody (CAB1731) is suitable for use in a range of applications, including Western blotting, immunofluorescence, and immunoprecipitation. It provides reliable and sensitive detection of XBP1, enabling researchers to further explore the functions and mechanisms of this important transcription factor.
Product Name:
XBP1 Rabbit Polyclonal Antibody
SKU:
CAB1731
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids1-70 of human XBP1 (NP_005071.2).
Cytoplasm, Endoplasmic reticulum membrane, Endoplasmic reticulum, Membrane, Nucleus, Peripheral membrane protein, Single-pass type II membrane protein.
Calculated MW:
29kDa
Observed MW:
60kDa
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Purification Method:
Affinity purification
Gene ID:
7494
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen, using XBP1 Rabbit pAb (CAB1731) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
