The XBP1 Polyclonal Antibody (CAB14796) is a valuable tool for researchers studying XBP1, a crucial transcription factor involved in the unfolded protein response (UPR) pathway. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the XBP1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and disease research.XBP1 is a key regulator of cellular stress responses, particularly in the endoplasmic reticulum, and is known to play a critical role in maintaining cellular homeostasis under adverse conditions.
Dysregulation of XBP1 has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and metabolic diseases. By studying the function of XBP1 using this antibody, researchers can gain valuable insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.
Product Name:
XBP1 Rabbit Polyclonal Antibody
SKU:
CAB14796
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human XBP1 (NP_005071.2).
Cytoplasm, Endoplasmic reticulum membrane, Endoplasmic reticulum, Membrane, Nucleus, Peripheral membrane protein, Single-pass type II membrane protein.
Calculated MW:
29kDa
Observed MW:
Refer to figures
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Purification Method:
Affinity purification
Gene ID:
7494
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunofluorescence analysis of U2OS cells using XBP1 antibody (CAB14796) at dilution of 1:100. Blue: DAPI for nuclear staining.
