The Xanthine Oxidase Rabbit Monoclonal Antibody (CAB9022) is a research tool optimized for the detection of xanthine oxidase, an enzyme involved in purine metabolism and the production of reactive oxygen species. This antibody, generated in rabbits, offers high specificity and sensitivity in detecting xanthine oxidase in samples from various species, making it an essential tool for studies in biochemistry, pharmacology, and oxidative stress research.Xanthine oxidase is a key enzyme in the metabolism of purines, playing a crucial role in the conversion of hypoxanthine to xanthine and xanthine to uric acid. Its activity has been linked to various pathological conditions, including gout, cardiovascular diseases, and inflammatory disorders.
By targeting xanthine oxidase with this antibody, researchers can gain valuable insights into the regulation of purine metabolism and the impact of reactive oxygen species on cellular function.This antibody is validated for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence, providing researchers with versatile options for studying the expression and localization of xanthine oxidase in different cell types and tissues. With its high specificity and sensitivity, the Xanthine Oxidase Rabbit Monoclonal Antibody (CAB9022) is a valuable tool for advancing our understanding of the roles of xanthine oxidase in health and disease.
Product Name:
Xanthine Oxidase (XDH) Rabbit Monoclonal Antibody
SKU:
CAB9022
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 202-293 of human Xanthine Oxidase (XDH) (P47989).
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
Purification Method:
Affinity purification
Gene ID:
7498
Clone Number:
ARC1385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Xanthine Oxidase (XDH) Rabbit mAb (CAB9022) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
