The WWP1 Polyclonal Antibody (CAB5269) is a vital tool for research involving the WWP1 protein, a key regulator of protein degradation pathways. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for use in Western blot applications. By binding to the WWP1 protein, researchers can effectively study protein turnover and degradation processes in various cell types, providing valuable insights into cellular function and pathway regulation.WWP1, a HECT-type E3 ubiquitin ligase, plays a crucial role in targeting specific proteins for ubiquitination and subsequent degradation, thus influencing various cellular processes such as cell cycle regulation, signal transduction, and DNA repair.
Dysregulation of WWP1 has been implicated in cancer development and progression, making it a promising target for cancer research and therapeutic intervention. By investigating the function of WWP1 with this polyclonal antibody, researchers can further unravel the complex mechanisms underlying protein degradation and its impact on cellular physiology.
Product Name:
WWP1 Rabbit Polyclonal Antibody
SKU:
CAB5269
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WWP1 (NP_008944.1).
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.
Purification Method:
Affinity purification
Gene ID:
11059
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse liver, using WWP1 antibody (CAB5269) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
