The WTAP Polyclonal Antibody is a versatile tool for researchers studying the WTAP protein, which plays a key role in mRNA processing and gene expression regulation. This antibody, produced in rabbits, shows high specificity for human samples and is optimized for use in Western blotting applications. By binding to WTAP, this antibody allows for precise detection and analysis of the protein in various cell types, making it an essential reagent for investigations in molecular biology and cancer research.
WTAP, also known as Wilms tumor 1-associated protein, is involved in various cellular processes, including RNA splicing and epigenetic regulation. Dysregulation of WTAP has been implicated in the development and progression of multiple cancer types, making it a promising therapeutic target for cancer treatment. By understanding the function of WTAP, researchers can uncover potential mechanisms for tumorigenesis and identify novel strategies for combating cancer progression.
Product Name:
WTAP Polyclonal Antibody
SKU:
CAB22004
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 212-355 of human WTAP (NP_004897.2).
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.
Purification Method:
Affinity purification
Gene ID:
9589
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of RAW264.7 cells, using WTAP antibody (CAB22004) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
