The WTAP Polyclonal Antibody (CAB14695) is a valuable tool for researchers studying the WTAP protein, involved in the regulation of RNA processing and gene expression. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the WTAP protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology and cancer research.
WTAP, also known as Wilms tumor 1 associated protein, plays a crucial role in RNA modification and is implicated in various cellular processes, including cell growth and differentiation. Its involvement in gene regulation makes it a key target for research into diseases such as cancer, where dysregulation of gene expression is common. Understanding the function of WTAP is essential for unraveling its role in disease development and potential therapeutic interventions aimed at targeting these processes.
Product Name:
WTAP Rabbit Polyclonal Antibody
SKU:
CAB14695
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 212-355 of human WTAP (NP_004897.2).
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.
Purification Method:
Affinity purification
Gene ID:
9589
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using WTAP antibody (CAB14695) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
