The WT1 Polyclonal Antibody (CAB16319) is a vital tool for research involving the Wilms tumor 1 (WT1) protein, a transcription factor with key roles in both normal development and cancer. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in a variety of applications including Western blotting.The WT1 protein is known to play crucial roles in cell growth, development, and differentiation, making it a significant target for investigations into various diseases, especially cancer. Its involvement in tumorigenesis and tumor progression has made it a promising biomarker for cancer diagnosis and prognosis.
Researchers studying WT1 will find this antibody to be essential for detecting and analyzing the protein in a range of cell types, aiding in the understanding of its functions and mechanisms of action. Its versatility and reliability make it an invaluable tool for studies in cancer biology and developmental biology, providing insights that may ultimately lead to improved diagnostic and therapeutic strategies for cancer patients.
Product Name:
WT1 Rabbit Polyclonal Antibody
SKU:
CAB16319
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 74-254 of human WT1 (NP_077744.4).
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Purification Method:
Affinity purification
Gene ID:
7490
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using WT1 Rabbit pAb (CAB16319) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
