WT1 Rabbit Polyclonal Antibody (CAB16319)
- SKU:
- CAB16319
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | WT1 Rabbit Polyclonal Antibody |
SKU: | CAB16319 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 74-254 of human WT1 (NP_077744.4). |
Sequence: | MGSD VRDL NALL PAVP SLGG GGGC ALPV SGAA QWAP VLDF APPG ASAY GSLG GPAP PPAP PPPP PPPP HSFI KQEP SWGG AEPH EEQC LSAF TVHF SGQF TGTA GACR YGPF GPPP PSQA SSGQ ARMF PNAP YLPS CLES QPAI RNQG YSTV TFDG TPSY GHTP SHHA AQFP NHSF KHED P |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
Synonyms: | GUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2; WT1 |
Positive Sample: | K-562,293T,OVCAR3,MCF-7,Mouse testis,Rat testis |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm, Nucleus, Nucleus speckle, nucleolus, nucleoplasm. |
Calculated MW: | 49kDa |
Observed MW: | 49kDa |
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Purification Method: | Affinity purification |
Gene ID: | 7490 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |