The WT1 Polyclonal Antibody (CAB16298) is a valuable tool for researchers studying the WT1 protein, which is known to play a crucial role in various cellular processes such as cell proliferation, differentiation, and apoptosis. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.WT1 is a transcription factor that is frequently overexpressed in various cancers, making it a potential target for cancer therapy.
By binding to the WT1 protein, this antibody enables the detection and analysis of WT1 expression in different cell types, providing valuable insights for cancer research and drug development.Furthermore, WT1 has been implicated in other diseases such as kidney and cardiovascular disorders, highlighting its importance beyond cancer biology. Studying the role of WT1 using this antibody can uncover new therapeutic targets and strategies for treating a wide range of diseases.
Product Name:
WT1 Rabbit Polyclonal Antibody
SKU:
CAB16298
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1).
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Purification Method:
Affinity purification
Gene ID:
7490
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using WT1 antibody (CAB16298) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
