The Wnt8A Monoclonal Antibody (PAC058472) provided by AssayGenie is a valuable tool for researchers studying the Wnt8A protein, a key player in the Wnt signaling pathway. This antibody is produced using state-of-the-art technology and is highly specific to Wnt8A, ensuring accurate and reliable results in various experimental settings.The Wnt signaling pathway is involved in numerous cellular processes, including cell proliferation, differentiation, and migration. Dysregulation of this pathway has been linked to various diseases, including cancer and developmental disorders.
The ability to accurately detect and measure Wnt8A expression is crucial for understanding its role in these diseases and identifying potential therapeutic targets.This Wnt8A Monoclonal Antibody is suitable for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence, allowing researchers to analyze Wnt8A expression levels and localization in different cell types and tissues. Its high specificity and sensitivity make it a valuable tool for both basic research and drug development efforts targeting the Wnt signaling pathway.
Antibody Name:
WNT8A Antibody (PACO58472)
Antibody SKU:
PACO58472
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Protein Wnt-8a protein (223-309AA)
IHC image of PACO58472 diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO58472 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Synonyms:
Protein Wnt-8a (Protein Wnt-8d), WNT8A, WNT8D
UniProt Protein Function:
WNT8A: Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Secreted; Secreted, signal peptide; Extracellular matrixChromosomal Location of Human Ortholog: 5q31Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular regionMolecular Function: frizzled binding; receptor agonist activityBiological Process: neuron differentiation; response to retinoic acid; neural crest cell fate commitment; palate development; Wnt receptor signaling pathway through beta-catenin
UniProt Protein Details:
NCBI Summary:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
