The Wnt3 Polyclonal Antibody (CAB9328) is a valuable tool for researchers studying the Wnt3 protein, a member of the Wnt signaling pathway involved in various cellular processes such as cell proliferation, differentiation, and development. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications.By specifically binding to the Wnt3 protein, this antibody allows for the detection and analysis of Wnt3 in a variety of cell types, making it ideal for investigations in developmental biology, cancer research, and regenerative medicine.
Understanding the role of Wnt3 in signaling pathways is crucial for unraveling its implications in various diseases and for potentially targeting it in therapeutic interventions.The Wnt3 Polyclonal Antibody (CAB9328) provides researchers with a reliable tool to study the function and regulation of the Wnt3 protein, paving the way for advancements in the understanding of cellular processes and disease mechanisms influenced by Wnt signaling.
Product Name:
WNT3 Rabbit Polyclonal Antibody
SKU:
CAB9328
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 166-355 of human WNT3 (NP_110380.1).
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.
Purification Method:
Affinity purification
Gene ID:
7473
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using WNT3 Rabbit pAb (CAB9328) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
