Anti-WNT1 Antibody (CAB2475)

Antibody Name:	Anti-WNT1 Antibody
Antibody SKU:	CAB2475
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-370 of human WNT1 (NP_005421.1).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:4000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HepG2, Mouse liver, Mouse heart, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-370 of human WNT1 (NP_005421.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TCWM RLPT LRAV GDVL RDRF DGAS RVLY GNRG SNRA SRAE LLRL EPED PAHK PPSP HDLV YFEK SPNF CTYS GRLG TAGT AGRA CNSS SPAL DGCE LLCC GRGH RTRT QRVT ERCN CTFH WCCH VSCR NCTH TRVL HECL
Gene ID:	7471
Uniprot:	P04628
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	40kDa
Observed MW:	49kDa

Synonyms:

WNT1, BMND16, INT1, OI15

Background:

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.

UniProt Protein Function:	WNT1: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Belongs to the Wnt family.
UniProt Protein Details:	Protein type:Cell development/differentiation; Secreted, signal peptide; Motility/polarity/chemotaxis; Oncoprotein; Secreted Chromosomal Location of Human Ortholog: 12q13 Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; Golgi lumen; cytoplasm; plasma membrane; extracellular region Molecular Function:protein domain specific binding; frizzled binding; cytokine activity; receptor agonist activity Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; ubiquitin-dependent SMAD protein catabolic process; diencephalon development; negative regulation of BMP signaling pathway; neuron differentiation; cerebellum formation; positive regulation of fibroblast proliferation; cell-cell signaling; midbrain development; positive regulation of cell proliferation; midbrain-hindbrain boundary maturation during brain development; response to wounding; positive regulation of Notch signaling pathway; myoblast fusion; neuron fate determination; organ regeneration; inner ear morphogenesis; Wnt receptor signaling pathway; cell fate commitment; embryonic axis specification; DNA damage response, signal transduction; negative regulation of fat cell differentiation; central nervous system morphogenesis; ureteric bud branching; forebrain anterior/posterior pattern formation; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; Spemann organizer formation Disease: Bone Mineral Density Quantitative Trait Locus 16; Osteogenesis Imperfecta, Type Xv
NCBI Summary:	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]
UniProt Code:	P04628
NCBI GenInfo Identifier:	139743
NCBI Gene ID:	7471
NCBI Accession:	P04628.1
UniProt Related Accession:	P04628
Molecular Weight:
NCBI Full Name:	Proto-oncogene Wnt-1
NCBI Synonym Full Names:	wingless-type MMTV integration site family, member 1
NCBI Official Symbol:	WNT1
NCBI Official Synonym Symbols:	INT1; OI15; BMND16
NCBI Protein Information:	proto-oncogene Wnt-1
UniProt Protein Name:	Proto-oncogene Wnt-1
UniProt Synonym Protein Names:	Proto-oncogene Int-1 homolog
Protein Family:	WNT1-inducible-signaling pathway protein
UniProt Gene Name:	WNT1

	Western blot analysis of extracts of various cell lines, using WNT1 antibody (CAB2475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using WNT1 antibody (CAB2475) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat liver using WNT1 antibody (CAB2475) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using WNT1 antibody (CAB2475) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
WNT1 Antibody (PACO06856)	Human WNT1 ELISA Kit
	Human Proto-oncogene Wnt-1 (WNT1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)