WNK4 Antibody (PACO46906)
- SKU:
- PACO46906
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
WNK4 Antibody (PACO46906)
The WNK4 Polyclonal Antibody (PAC046906) is a crucial tool for research involving WNK4, a protein kinase that plays a key role in regulating blood pressure and electrolyte balance in the body. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as Western blotting and immunohistochemistry. By targeting the WNK4 protein, researchers are able to study its expression and function in different cell types, allowing for a better understanding of its involvement in physiological processes related to cardiovascular health and kidney function.
WNK4 is known for its important function in the regulation of ion transport in the kidney and other tissues, making it a target of interest for studies related to hypertension, electrolyte disorders, and kidney disease. By investigating the role of WNK4 in these conditions, researchers may uncover new therapeutic strategies or diagnostic markers that could improve patient outcomes. The WNK4 Polyclonal Antibody provides a valuable tool for researchers seeking to explore the molecular mechanisms underlying these health conditions.
| Antibody Name: | WNK4 Antibody (PACO46906) |
| Antibody SKU: | PACO46906 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Serine/threonine-protein kinase WNK4 protein (828-1106AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunohistochemistry of paraffin-embedded human kidney tissue using PACO46906 at dilution of 1:100. |
 | Immunofluorescent analysis of PC-3 cells using PACO46906 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunofluorescent analysis of HepG2 cells using PACO46906 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity). |
| Synonyms: | Serine/threonine-protein kinase WNK4 (EC 2.7.11.1) (Protein kinase lysine-deficient 4) (Protein kinase with no lysine 4), WNK4, PRKWNK4 |
| UniProt Protein Function: | WNK4: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Interacts with the C-terminal region of KCNJ1. Interacts with WNK1 and WNK3. Expressed in kidney, colon and skin. Activation requires autophosphorylation of Ser- 335. Phosphorylation of Ser-331 also promotes increased activity. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Other group; Wnk familyChromosomal Location of Human Ortholog: 17q21-q22Cellular Component: tight junction; cytoplasmMolecular Function: protein serine/threonine kinase activity; protein binding; chloride channel inhibitor activity; ATP bindingBiological Process: protein localization; ion homeostasis; regulation of cellular process; ion transport; chloride transport; protein amino acid phosphorylationDisease: Pseudohypoaldosteronism, Type Iib |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q96J92 |
| NCBI GenInfo Identifier: | 34365793 |
| NCBI Gene ID: | 65266 |
| NCBI Accession: | NP_115763.2 |
| UniProt Secondary Accession: | Q96J92,Q8N8X3, Q8N8Z2, Q96DT8, Q9BYS5, B0LPI0 |
| UniProt Related Accession: | Q96J92 |
| Molecular Weight: | 134,739 Da |
| NCBI Full Name: | serine/threonine-protein kinase WNK4 |
| NCBI Synonym Full Names: | WNK lysine deficient protein kinase 4 |
| NCBI Official Symbol: | WNK4Â Â |
| NCBI Official Synonym Symbols: | PHA2B; PRKWNK4Â Â |
| NCBI Protein Information: | serine/threonine-protein kinase WNK4; protein kinase with no lysine 4; protein kinase lysine-deficient 4 |
| UniProt Protein Name: | Serine/threonine-protein kinase WNK4 |
| UniProt Synonym Protein Names: | Protein kinase lysine-deficient 4; Protein kinase with no lysine 4 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | WNK4Â Â |
| UniProt Entry Name: | WNK4_HUMAN |
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