The WNK3 Polyclonal Antibody (PAC020909) is a valuable tool for researchers studying WNK3, a serine/threonine protein kinase that plays a critical role in regulating ion transport and blood pressure. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds specifically to the WNK3 protein, allowing for precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of physiology, biochemistry, and cardiology.WNK3 is a key regulator of ion transport in the kidney, colon, and blood vessels, influencing processes such as sodium and potassium balance, blood pressure control, and cell volume regulation.
Dysregulation of WNK3 activity has been linked to diseases like hypertension, electrolyte imbalance, and cardiovascular disorders, highlighting the importance of understanding its molecular function. Research on WNK3 can provide valuable insights into the pathogenesis of these conditions and potentially lead to the development of novel therapeutic strategies targeting WNK3 signaling pathways.
Antibody Name:
WNK3 Antibody (PACO20909)
Antibody SKU:
PACO20909
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:10-1:50
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human WNK3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20909(WNK3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20909(WNK3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.
Synonyms:
WNK lysine deficient protein kinase 3
UniProt Protein Function:
WNK3: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; Wnk family
Molecular Function:ATP binding; chloride channel inhibitor activity; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: negative regulation of apoptosis; positive regulation of calcium ion transport; positive regulation of ion transmembrane transporter activity; protein amino acid autophosphorylation; protein amino acid phosphorylation
NCBI Summary:
This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
