The WIPI2 Polyclonal Antibody (CAB7537) is a critical tool for researchers studying WIPI2, a key protein involved in autophagy, a cellular process essential for maintaining cellular homeostasis and responding to stress. This antibody, produced in rabbits, is highly specific to human WIPI2 and has been validated for use in Western blot applications. By binding to WIPI2, this antibody allows for the detection and analysis of WIPI2 expression in a wide range of cell types, making it an invaluable tool for autophagy research and the study of related diseases.
WIPI2, also known as WD Repeat Domain, phosphoinositide Interacting 2, is involved in the formation of autophagosomes, the vesicles responsible for delivering cellular components to lysosomes for degradation. Dysregulation of autophagy, including WIPI2 function, has been implicated in various diseases, including neurodegenerative disorders, cancer, and metabolic diseases. Understanding the role of WIPI2 in autophagy is essential for developing targeted therapies to modulate this process and potentially treat these diseases effectively.
Product Name:
WIPI2 Rabbit Polyclonal Antibody
SKU:
CAB7537
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human WIPI2 (NP_057087.2).
WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).
Purification Method:
Affinity purification
Gene ID:
26100
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using WIPI2 antibody (CAB7537) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
