The WIPF2 Polyclonal Antibody (PAC059249) is a valuable tool for researchers studying WIPF2, a protein involved in regulating cell migration and cytoskeletal organization. This antibody, produced in rabbits, shows high specificity for human samples and is validated for use in Western blot applications. By targeting the WIPF2 protein, this antibody allows for precise detection and analysis in various cell types, making it an essential component for studies in cell biology and cancer research.WIPF2, also known as WAVE2, plays a crucial role in controlling cell movement and shape changes by promoting the assembly of actin filaments.
Its involvement in cytoskeletal dynamics makes it a key player in processes such as cell migration, immune cell function, and cancer metastasis. Understanding the function of WIPF2 is paramount for developing novel strategies to interfere with aberrant cell behaviors in diseases like cancer and inflammatory disorders.Overall, the WIPF2 Polyclonal Antibody is a critical tool for researchers looking to delve into the molecular mechanisms of cell movement and cytoskeletal regulation, opening up new possibilities for therapeutic interventions in various pathological conditions.
Antibody Name:
WIPF2 Antibody (PACO59249)
Antibody SKU:
PACO59249
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:500-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human WAS/WASL-interacting protein family member 2 protein (18-176AA)
IHC image of PACO59249 diluted at 1:500 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO59249 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system.
Synonyms:
WAS/WASL-interacting protein family member 2 (WASP-interacting protein-related protein) (WIP- and CR16-homologous protein) (WIP-related protein), WIPF2, WICH WIRE
UniProt Protein Function:
WIRE: Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system. Belongs to the verprolin family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffoldChromosomal Location of Human Ortholog: 17q21.2Cellular Component: actin filament; cytosolMolecular Function: actin filament binding; protein bindingBiological Process: actin cortical patch assembly; actin cortical patch localization; actin filament-based movement; endocytosis; positive regulation of actin nucleation; regulation of cell shape
UniProt Protein Details:
NCBI Summary:
This gene encodes a WASP interacting protein (WIP)-related protein. It has been shown that this protein has a role in the WASP-mediated organization of the actin cytoskeleton and that this protein is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery. [provided by RefSeq, Jul 2008]
