The Wilms Tumor Protein Polyclonal Antibody (CAB1198) is a valuable tool for researchers studying the Wilms tumor protein (WT1), a transcription factor involved in cell growth and development. This antibody, raised in rabbits, is highly specific for WT1 and has been validated for use in various applications, including Western blotting and immunohistochemistry.WT1 is known for its role in the development of Wilms tumor, a type of kidney cancer that primarily affects children. Additionally, WT1 has been implicated in other cancers, such as leukemia and mesothelioma, making it a target of interest for cancer research.
By using the Wilms Tumor Protein Polyclonal Antibody, researchers can detect and analyze WT1 expression in various cell types and tissues, providing valuable insights into its function and potential as a therapeutic target. This antibody is essential for studies investigating the molecular mechanisms underlying cancer development and progression, ultimately contributing to the advancement of diagnostic and treatment strategies for cancer patients.
Product Name:
WT1 Rabbit Polyclonal Antibody
SKU:
CAB1198
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1).
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Purification Method:
Affinity purification
Gene ID:
7490
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using WT1 antibody (CAB1198) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
