The WIF1 Rabbit Monoclonal Antibody (CAB5171) is a valuable tool for research involving WIF1, a secreted protein known to act as a Wnt signaling pathway antagonist. The antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting WIF1 in human samples and is validated for use in various applications, including Western blotting.WIF1 is a crucial player in regulating the Wnt signaling pathway, which is involved in various cellular processes such as cell proliferation, differentiation, and migration. Dysregulation of Wnt signaling has been implicated in numerous diseases, including cancer and developmental disorders.
The ability to accurately detect and quantify WIF1 levels in different cell types makes this antibody an essential resource for researchers studying Wnt signaling and its impact on disease progression.By targeting WIF1, researchers can gain valuable insights into the mechanisms underlying disease development and potentially identify novel therapeutic targets for conditions influenced by aberrant Wnt signaling. This antibody is a valuable addition to any immunology or cancer research laboratory seeking to further understand the role of WIF1 in health and disease.
Product Name:
WIF1 Rabbit Monoclonal Antibody
SKU:
CAB5171
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 280-379 of human WIF1 (Q9Y5W5).
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Purification Method:
Affinity purification
Gene ID:
11197
Clone Number:
ARC1161
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using WIF1 Rabbit mAb (CAB5171) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
