The WIF1 Polyclonal Antibody (CAB5386) is a valuable tool for researchers studying the Wnt inhibitory factor 1 (WIF1) protein, which is involved in the regulation of the Wnt signaling pathway. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications.WIF1 is known to function as a tumor suppressor, inhibiting cell proliferation and promoting apoptosis in various cancer types. Its role in regulating cell growth and differentiation makes it a promising target for cancer research and drug development.
By targeting WIF1, researchers aim to better understand the mechanisms underlying tumorigenesis and potentially develop new therapeutic strategies.The WIF1 Polyclonal Antibody binds specifically to the WIF1 protein, allowing for the detection and analysis of WIF1 expression in different cell types and tissues. Its versatility and reliability make it a valuable tool for studies in oncology, developmental biology, and regenerative medicine. Researchers interested in exploring the role of WIF1 in health and disease will find this antibody essential for their experiments.
Product Name:
WIF1 Rabbit Polyclonal Antibody
SKU:
CAB5386
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 307-377 of human WIF1 (NP_009122.2).
Sequence:
KPVC EPGC GAHG TCHE PNKC QCQE GWHG RHCN KRYE ASLI HALR PAGA QLRQ HTPS LKKA EERR DPPE SNY
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:100 - 1:500 IF/ICC,1:50 - 1:200
Synonyms:
WIF-1; WIF1
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
42kDa
Observed MW:
42kDa
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Purification Method:
Affinity purification
Gene ID:
11197
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HeLa, using WIF1 antibody (CAB5386) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
