The Wif1 Polyclonal Antibody (CAB12969) is a valuable tool for researchers studying the Wif1 protein, which plays a key role in regulating Wnt signaling pathways. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically targeting the Wif1 protein, this antibody enables precise detection and analysis in various cell types, making it an essential tool for studies in developmental biology, cancer research, and regenerative medicine.
The Wif1 protein functions as a secreted antagonist of Wnt signaling, a critical pathway involved in cell proliferation, differentiation, and development. Dysregulation of Wnt signaling is implicated in various diseases, including cancer, developmental disorders, and degenerative diseases. By studying the role of Wif1 in modulating Wnt signaling, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
Product Name:
WIF1 Rabbit Polyclonal Antibody
SKU:
CAB12969
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-220 of human WIF1 (NP_009122.2).
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Purification Method:
Affinity purification
Gene ID:
11197
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with WIF1 using WIF1 Rabbit pAb (CAB12969) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
