The NSD3/WHSC1L1 Antibody (CAB5577) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. RRID AB_2766354 Gene ID 54904 Swiss Prot Synonym KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328; L1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NSD3/WHSC1L1 Antibody
SKU:
CAB5577
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328, L1
Positive Sample:
293T
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
162kDa
Observed MW:
180kDa
This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. RRID AB_2766354 Gene ID 54904 Swiss Prot Synonym KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328; L1
Purification Method:
Affinity purification
Gene ID:
54904
RRID:
AB_2766354
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] NSD3/WHSC1L1 Rabbit pAb (CAB5577) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and NSD3/WHSC1L1 knockout (KO) 293T cells, using [KO Validated] NSD3/WHSC1L1 Rabbit pAb (CAB5577) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
