The Wee1 Polyclonal Antibody (CAB16256) is a valuable tool for researchers studying the protein Wee1, a key regulator of the cell cycle and DNA replication. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the Wee1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and cancer research.
Wee1 is an essential protein involved in controlling the G2/M checkpoint in the cell cycle, preventing premature entry into mitosis and ensuring proper DNA replication. Dysregulation of Wee1 has been implicated in various cancers, making it a promising target for therapeutic intervention. Research into the function and regulation of Wee1 is crucial for understanding the mechanisms underlying cancer development and identifying potential treatment strategies.
Product Name:
WEE1 Rabbit Polyclonal Antibody
SKU:
CAB16256
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human WEE1 (NP_003381.1).
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Purification Method:
Affinity purification
Gene ID:
7465
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using WEE1 Rabbit pAb (CAB16256) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
