The WDR59 Antibody (PAC063143) is a highly specific polyclonal antibody designed for research involving the WDR59 protein. This antibody, raised in rabbits, is known to be highly reactive with human samples and has been validated for use in various applications, including Western blotting. By binding to the WDR59 protein, this antibody enables accurate detection and analysis in different cell types, making it an excellent tool for studies in the fields of molecular biology and cell biology.WDR59, also known as WD repeat-containing protein 59, is a critical player in various cellular processes such as transcription regulation, intracellular trafficking, and cell cycle progression.
Understanding the function and regulation of WDR59 is essential for unraveling its role in normal cellular function and its potential involvement in diseases such as cancer and neurological disorders. Research using the WDR59 Antibody can provide valuable insights into the complex molecular mechanisms involving WDR59, contributing to advancements in biomedical research and potential therapeutic interventions.
Immunofluorescence staining of U251 cells with PACO63143 at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot. Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate. All lanes: WDR59 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 110, 65, 63, 47 kDa. Observed band size: 110 kDa.
Background:
As a component of the GATOR subcomplex GATOR2, functions within the amino acid, sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid, sensors SESN2 and CASTOR1.
Synonyms:
GATOR complex protein WDR59 (WD repeat-containing protein 59), WDR59, KIAA1923
UniProt Protein Function:
WDR59: Belongs to the WD repeat WDR59 family. 4 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 16q23.1Molecular Function: protein bindingBiological Process: cellular response to amino acid starvation; positive regulation of TOR signaling pathway
