The WDFY3 Polyclonal Antibody (PACO58260) is a valuable tool for researchers studying WDFY3, a protein involved in cellular signaling pathways and autophagy regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting WDFY3 in human samples, making it an ideal choice for Western blot applications. By binding to the WDFY3 protein, this antibody enables accurate detection and analysis in a variety of cell types, facilitating research in fields such as cell biology, molecular biology, and disease mechanisms.WDFY3, also known as WD repeat and FYVE domain-containing protein 3, plays a significant role in intracellular trafficking and membrane dynamics, with implications for cellular homeostasis and organelle function.
Its involvement in autophagy pathways suggests potential relevance to diseases such as neurodegenerative disorders, metabolic conditions, and cancer. Investigating the functions of WDFY3 with this antibody can provide valuable insights into the molecular mechanisms underlying these diseases and may offer new therapeutic targets for future drug development efforts.
Immunohistochemistry of paraffin-embedded human gastric cancer using PACO58260 at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PACO58260 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human colon cancer using PACO58260 at dilution of 1:100.
Background:
Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.
Synonyms:
WD repeat and FYVE domain-containing protein 3 (Autophagy-linked FYVE protein) (Alfy), WDFY3, KIAA0993
UniProt Protein Function:
WDFY3: 2 isoforms of the human protein are produced by alternative splicing.Protein type: Lipid-binding; Nuclear envelope; Membrane protein, peripheral; TransferaseChromosomal Location of Human Ortholog: 4q21.23Cellular Component: PML body; nuclear membrane; extrinsic to membrane; cytoplasm; autophagic vacuole; nuclear envelope; inclusion bodyMolecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; protein binding; metal ion binding; phosphatidylinositol bindingBiological Process: endosome transport; positive regulation of macroautophagy
UniProt Protein Details:
NCBI Summary:
This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]
