The WDFY1 Polyclonal Antibody (PAC049234) is a valuable tool for researchers studying WDFY1, a protein involved in autophagy and immune regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the WDFY1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in immunology and cancer research.WDFY1, also known as WD repeat and FYVE domain-containing protein 1, plays a key role in autophagosome formation and cargo recognition, thereby influencing cellular homeostasis and immune responses.
The importance of WDFY1 in these processes positions it as a promising target for research on diseases such as cancer, neurodegenerative disorders, and infectious diseases. By studying the function of WDFY1, researchers can uncover new insights into disease mechanisms and develop innovative therapeutic strategies aimed at manipulating autophagy and immune responses.
Antibody Name:
WDFY1 Antibody (PACO49234)
Antibody SKU:
PACO49234
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant Human WD repeat and FYVE domain-containing protein 1 protein (261-357AA)
Western Blot. Positive WB detected in: Mouse liver tissue, Mouse kidney tissue. All lanes: WDFY1 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47 kDa. Observed band size: 47 kDa..
Background:
Positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-β and inflammatory cytokines.
Synonyms:
WD repeat and FYVE domain-containing protein 1 (FENS-1) (Phosphoinositide-binding protein 1) (WD40- and FYVE domain-containing protein 1) (Zinc finger FYVE domain-containing protein 17), WDFY1, KIAA1435 WDF1 ZFYVE17
UniProt Protein Function:
WDFY1: Binds PtdIns3P in vitro with high specificity over other phosphoinositides.Chromosomal Location of Human Ortholog: 2q36.1Cellular Component: cytosol; early endosome; nucleusMolecular Function: phosphatidylinositol binding; protein bindingBiological Process: positive regulation of toll-like receptor 3 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain. [provided by RefSeq, Jan 2015]
