The VPX Antibody (PAC061842) is a valuable tool for researchers studying the VPX protein, a key component in HIV-2 and SIV viral replication. This polyclonal antibody, produced through rabbit immunization, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the VPX protein, this antibody enables the detection and analysis of VPX in various cell types, making it a crucial resource for studies in virology and HIV/AIDS research.
The VPX protein is essential for efficient viral replication in HIV-2 and SIV, making it a promising target for antiviral drug development and therapy. Understanding the role of VPX in viral replication and pathogenesis is vital for advancing our knowledge of HIV/AIDS and developing effective treatments against these deadly viruses. The VPX Antibody (PAC061842) provides researchers with a powerful tool to investigate the function of VPX and its potential as a target for antiviral interventions.
Antibody Name:
vpx Antibody (PACO61842)
Antibody SKU:
PACO61842
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:
Human immunodeficiency virus type 2 subtype A
Immunogen:
Recombinant Human immunodeficiency virus type 2 subtype A Protein Vpx protein (1-112AA)
Western Blot. Positive WB detected in Recombinant protein. All lanes: vpx at 2.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa.
Background:
Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity).
Synonyms:
Protein Vpx (Viral protein X) (X ORF protein), vpx
UniProt Protein Function:
Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription ().
