The VPS54 Polyclonal Antibody (CAB13215) is a valuable tool for researchers studying VPS54, a key protein involved in intracellular trafficking and membrane fusion processes. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the VPS54 protein, this antibody enables the detection and analysis of VPS54 in various cell types, making it ideal for investigations in cell biology and molecular biology research.VPS54 is a crucial component of the Golgi-associated retrograde protein (GARP) complex, which plays a vital role in the retrograde transport of proteins from endosomes to the trans-Golgi network.
Dysregulation of VPS54 has been linked to various diseases, including neurodevelopmental disorders and neurodegenerative diseases. By studying the function and regulation of VPS54, researchers can gain insights into fundamental cellular processes and potentially develop novel therapeutic interventions for related disorders.Overall, the VPS54 Polyclonal Antibody (CAB13215) is a reliable tool for investigating the role of VPS54 in cellular pathways and disease processes. Its high specificity and versatility make it a valuable asset for researchers seeking to deepen their understanding of intracellular trafficking mechanisms and uncover new therapeutic targets.
Product Name:
VPS54 Rabbit Polyclonal Antibody
SKU:
CAB13215
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 420-720 of human VPS54 (NP_001005739.1).
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms
Purification Method:
Affinity purification
Gene ID:
51542
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS54 Rabbit pAb (CAB13215) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
