The VPS4B Polyclonal Antibody (CAB15758) is a valuable tool for researchers studying VPS4B, a protein involved in the endosomal sorting complex required for transport (ESCRT) machinery. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting VPS4B in human samples. Validated for use in Western blot applications, this antibody binds to VPS4B, enabling precise detection and analysis in a variety of cell types.VPS4B is essential for membrane remodeling and vesicle trafficking, playing a crucial role in various cellular processes such as multivesicular body formation and cytokinesis.
Dysregulation of VPS4B has been implicated in diseases including neurodegenerative disorders and viral infections. By studying the function of VPS4B, researchers can gain insights into these pathological conditions and potentially develop targeted therapies.Overall, the VPS4B Polyclonal Antibody is a reliable tool for investigating the role of VPS4B in cellular processes and disease pathogenesis, making it an invaluable asset for studies in cell biology, molecular biology, and biomedicine.
Product Name:
VPS4B Rabbit Polyclonal Antibody
SKU:
CAB15758
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human VPS4B (NP_004860.2).
Late endosome membrane, Peripheral membrane protein, Prevacuolar compartment membrane.
Calculated MW:
49kDa
Observed MW:
48kDa
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
Purification Method:
Affinity purification
Gene ID:
9525
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS4B Rabbit pAb (CAB15758) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
