The VPS4A Polyclonal Antibody (CAB7096) is a valuable tool for researchers studying VPS4A, a protein involved in the endosomal sorting complex required for transport (ESCRT) pathway. This antibody, produced in rabbits, shows high specificity for human samples and has been validated for use in Western blot applications.VPS4A is a key regulator of membrane remodeling and vesicle trafficking, playing a crucial role in various cellular processes such as multivesicular body biogenesis and viral budding. Research on VPS4A is essential for understanding its functions in protein sorting and degradation, as well as its implications in diseases like neurodegenerative disorders and cancer.
By targeting the VPS4A protein, this antibody allows for precise detection and analysis in different cell types, making it an ideal choice for studies in cell biology, molecular biology, and virology. With its high specificity and reliability, the VPS4A Polyclonal Antibody (CAB7096) is a valuable asset for researchers investigating the ESCRT pathway and its role in cellular homeostasis and disease development.
Product Name:
VPS4A Rabbit Polyclonal Antibody
SKU:
CAB7096
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-120 of human VPS4A (NP_037377.1).
Late endosome membrane, Midbody, Peripheral membrane protein, Prevacuolar compartment membrane.
Calculated MW:
49kDa
Observed MW:
49kDa
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18.
Purification Method:
Affinity purification
Gene ID:
27183
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS4A Rabbit pAb (CAB7096) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
