The VPS35 Rabbit Monoclonal Antibody (CAB9278) is a cutting-edge tool for researchers studying VPS35, a key player in the retromer complex involved in endosomal sorting and protein trafficking. This antibody, generated from rabbit cells, exhibits high specificity and sensitivity towards VPS35 in human samples, making it an essential tool for Western blot applications. By binding to the VPS35 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in cell biology and neurodegenerative diseases.VPS35 is crucial for maintaining cellular homeostasis by regulating the recycling of proteins from endosomes to the trans-Golgi network, impacting cell signaling, vesicle trafficking, and autophagy.
Dysregulation of VPS35 function has been linked to neurodegenerative disorders such as Parkinson's disease and Alzheimer's disease, highlighting its significance in understanding disease mechanisms and developing potential therapeutic strategies. The VPS35 Rabbit Monoclonal Antibody (CAB9278) provides researchers with a valuable tool to explore the roles of VPS35 in health and disease, advancing our understanding of cellular processes and potential treatment targets.
Product Name:
VPS35 Rabbit Monoclonal Antibody
SKU:
CAB9278
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human VPS35 (Q96QK1).
Cytoplasm, Early endosome, Endosome, Late endosome, Membrane, Peripheral membrane protein
Calculated MW:
92kDa
Observed MW:
92kDa
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Purification Method:
Affinity purification
Gene ID:
55737
Clone Number:
ARC1509
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using VPS35 Rabbit mAb (CAB9278) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
