The VPS35 Polyclonal Antibody (CAB7117) is a vital tool for researchers studying VPS35, a key component of the retromer complex involved in endosome-to-Golgi retrieval of transmembrane proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications.VPS35 plays a crucial role in cellular protein trafficking and sorting, making it essential for maintaining proper cellular function. Dysregulation of VPS35 has been implicated in various neurodegenerative disorders, including Parkinson's disease and Alzheimer's disease, highlighting its importance in understanding disease mechanisms.
By using the VPS35 Polyclonal Antibody, researchers can accurately detect and analyze VPS35 levels in different cell types, facilitating investigations into its role in disease pathology and potential therapeutic interventions. This antibody is a valuable tool for advancing research in neuroscience, cell biology, and neurodegenerative diseases.
Product Name:
VPS35 Rabbit Polyclonal Antibody
SKU:
CAB7117
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35 (NP_060676.2).
Cytoplasm, Early endosome, Endosome, Late endosome, Membrane, Peripheral membrane protein.
Calculated MW:
92kDa
Observed MW:
92kDa
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Purification Method:
Affinity purification
Gene ID:
55737
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using VPS35 antibody (CAB7117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.
