The VPS33B Polyclonal Antibody (CAB8799) is a valuable tool for researchers studying the VPS33B protein, which plays a crucial role in intracellular protein trafficking and membrane fusion processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.VPS33B is a key component of the Class C core vacuole/endosome tethering (CORVET) complex, which is involved in endosome fusion events and protein sorting pathways. Dysregulation of VPS33B has been implicated in various diseases, including neurodegenerative disorders and lysosomal storage diseases.
By detecting and analyzing VPS33B in different cell types, this antibody enables researchers to gain insights into the molecular mechanisms underlying these conditions and may lead to the development of novel therapeutic strategies. Its specificity and reliability make it an essential tool for studies in cell biology, neuroscience, and rare genetic diseases associated with VPS33B dysfunction.
Product Name:
VPS33B Rabbit Polyclonal Antibody
SKU:
CAB8799
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 348-617 of human VPS33B (NP_061138.3).
Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Late endosome membrane, Lysosome membrane, Peripheral membrane protein, Recycling endosome, clathrin-coated vesicle.
Calculated MW:
71kDa
Observed MW:
62kDa
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
26276
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using VPS33B antibody (CAB8799) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
