The VPS33A Antibody (PAC050890) is a polyclonal antibody designed for research involving VPS33A, a protein involved in regulating membrane trafficking and fusion in cells. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in various applications, including Western blot and immunofluorescence.VPS33A plays a crucial role in vesicle trafficking and organelle biogenesis, making it a key target for studying cellular processes such as autophagy and endocytosis. By targeting the VPS33A protein, researchers can gain insights into the mechanisms underlying these processes and their dysregulation in diseases such as neurodegenerative disorders and lysosomal storage diseases.
With its high reactivity and specificity, the VPS33A Antibody is a valuable tool for investigating the role of VPS33A in cell biology and disease pathology. Its potential applications in research areas such as cell biology, neurology, and rare genetic disorders make it essential for studies aiming to elucidate the molecular mechanisms governing membrane trafficking and organelle dynamics.
Immunohistochemistry of paraffin-embedded human liver cancer using PACO50890 at dilution of 1:100.
Immunofluorescent analysis of U251 cells using PACO50890 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.
Synonyms:
Vacuolar protein sorting-associated protein 33A (hVPS33A), VPS33A
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]
