The VPS33A Antibody (PAC017441) is a polyclonal antibody specifically designed for research involving VPS33A, a protein involved in membrane trafficking and fusion within cells. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the VPS33A protein, it allows for the detection and analysis of VPS33A in various cell types, making it an ideal tool for studies in cell biology and molecular biology.VPS33A is a key component of the HOPS complex, which plays a crucial role in intracellular membrane trafficking processes such as endosome-lysosome fusion and autophagy.
Dysregulation of VPS33A has been implicated in various diseases including lysosomal storage disorders and neurodegenerative disorders. Understanding the function of VPS33A is essential for unraveling the molecular mechanisms underlying these diseases and exploring potential therapeutic interventions.Overall, the VPS33A Antibody (PAC017441) offers researchers a valuable tool for studying the role of VPS33A in cellular processes and disease pathogenesis, paving the way for new insights and advancements in the field of cell biology and molecular biology.
Antibody Name:
VPS33A Antibody (PACO17441)
Antibody SKU:
PACO17441
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human VPS33A
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO17441(VPS33A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17441(VPS33A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
Synonyms:
vacuolar protein sorting 33 homolog A (S. cerevisiae)
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]
