The VPS33A Antibody (PAC017440) is a valuable tool for researchers studying VPS33A, a key protein involved in intracellular protein trafficking and vesicle fusion processes. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications.VPS33A is a critical component of the homotypic fusion and protein sorting (HOPS) complex, which plays a crucial role in endosome-lysosome fusion and autophagosome-lysosome fusion processes. Dysregulation of VPS33A function has been linked to various neurodegenerative disorders and lysosomal storage diseases.
By targeting VPS33A with this antibody, researchers can gain insights into the molecular mechanisms underlying these diseases and identify potential therapeutic targets.With its high reactivity and specificity, the VPS33A Antibody (PAC017440) is a valuable tool for investigating the role of VPS33A in cellular processes and disease pathogenesis. Its use in Western blot applications enables accurate detection and quantification of VPS33A protein levels in various cell types, making it an indispensable resource for studies in cell biology, neurology, and lysosomal storage disorders.
Antibody Name:
VPS33A Antibody (PACO17440)
Antibody SKU:
PACO17440
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human VPS33A
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO17440(VPS33A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17440(VPS33A Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
Synonyms:
vacuolar protein sorting 33 homolog A (S. cerevisiae)
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]
