The VPS29 Polyclonal Antibody (CAB13098) is a vital tool for researchers studying VPS29, a key component of retromer complex involved in protein trafficking and sorting within cells. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By binding to the VPS29 protein, this antibody allows for the detection and analysis of VPS29 in various cell types, facilitating research in cell biology and neurodegenerative diseases.
VPS29, also referred to as vacuolar protein sorting-associated protein 29, plays a crucial role in intracellular vesicle trafficking and protein sorting processes. Dysregulation of VPS29 has been implicated in neurodegenerative disorders such as Parkinson's disease and Alzheimer's disease, making it a promising target for therapeutic interventions. Understanding the function and regulation of VPS29 is essential for developing treatments that could potentially mitigate the progression of these debilitating diseases.
Product Name:
VPS29 Rabbit Polyclonal Antibody
SKU:
CAB13098
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human VPS29 (NP_476528.1).
Cytoplasm, Early endosome, Endosome membrane, Late endosome, Membrane, Peripheral membrane protein.
Calculated MW:
21kDa
Observed MW:
20kDa
This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
51699
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS29 Rabbit pAb (CAB13098) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
