The VPS28 Polyclonal Antibody (CAB9104) is a vital tool for researchers studying VPS28, a key component of the endosomal sorting complex required for transport (ESCRT) machinery involved in protein trafficking and membrane remodeling. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications.VPS28 plays a crucial role in the sorting of ubiquitinated cargo proteins and is essential for multivesicular body (MVB) biogenesis and lysosomal degradation. Dysregulation of VPS28 has been linked to various diseases, including neurodegenerative disorders and viral infections, underscoring its importance in cellular homeostasis.
By detecting and analyzing VPS28 protein in different cell types, the VPS28 Polyclonal Antibody enables researchers to gain insights into the mechanisms underlying protein sorting and trafficking processes. Its utility in immunology, cell biology, and disease research makes it an invaluable tool for further understanding the intricate workings of the ESCRT machinery and its implications in human health.
Product Name:
VPS28 Rabbit Polyclonal Antibody
SKU:
CAB9104
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human VPS28 (NP_057292.1).
Cell membrane, Late endosome membrane, Peripheral membrane protein.
Calculated MW:
25kDa
Observed MW:
25kDa
This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
51160
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS28 Rabbit pAb (CAB9104) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
