The VPS26A Polyclonal Antibody (PAC020865) is a valuable tool for research involving VPS26A, a key component of the retromer complex involved in protein trafficking and sorting within cells. This antibody, generated in rabbits, has been shown to have high reactivity with human samples and is validated for use in Western blot applications. By targeting the VPS26A protein, researchers can detect and analyze its expression in a variety of cell types, making it well-suited for studies in cell biology and molecular biology.VPS26A plays a crucial role in maintaining cellular homeostasis by facilitating the transport of proteins within cells, thereby influencing processes such as receptor recycling, signaling, and organelle function.
Dysregulation of VPS26A function has been implicated in various diseases, including neurodegenerative disorders, metabolic diseases, and infectious diseases. By studying VPS26A and its interactions, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO20865(VPS26A Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 12%SDS-PAGE,Lysate: 40 µg,Lane 1-7: Human placenta tissue, PC3 cells, Human fetal liver tissue, HepG2 cells, Hela cells, A431 cells, 293T cells,Primary antibody: PACO20865(VPS26A Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 40 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO20865(VPS26A Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms.
Synonyms:
vacuolar protein sorting 26 homolog A (S. pombe)
UniProt Protein Function:
VPS26A: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Belongs to the VPS26 family.
Molecular Function:protein binding; protein transporter activity
Biological Process: intracellular protein transport; regulation of macroautophagy; retrograde transport, endosome to Golgi; Wnt receptor signaling pathway
NCBI Summary:
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
