The VPS25 Polyclonal Antibody (CAB13132) is a valuable tool for researchers investigating VPS25, a key component of the endosomal sorting complex required for transport (ESCRT). This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the VPS25 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in cell biology, vesicle trafficking, and protein sorting mechanisms.VPS25 plays a crucial role in the ESCRT machinery, responsible for sorting and trafficking proteins within the cell.
Dysfunction in the ESCRT pathway has been linked to a variety of diseases, including viral infections, neurodegenerative disorders, and cancer. Research into the function and regulation of VPS25 is essential for understanding these pathological processes and developing targeted therapies.By utilizing the VPS25 Polyclonal Antibody, researchers can gain insights into the mechanisms of protein sorting and trafficking, as well as the role of VPS25 in cellular homeostasis. This antibody is a valuable tool for investigating the ESCRT pathway and its implications for a range of physiological and pathological processes.
Product Name:
VPS25 Rabbit Polyclonal Antibody
SKU:
CAB13132
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-176 of human VPS25 (NP_115729.1).
This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1.
Purification Method:
Affinity purification
Gene ID:
84313
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS25 Rabbit pAb (CAB13132) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
