The VPS16 Polyclonal Antibody (CAB15888) is a valuable tool for studying the Vacuolar Protein Sorting 16 homolog (VPS16) protein, which plays a key role in endosomal sorting and membrane trafficking processes within cells. This antibody is produced using rabbits and is highly specific to human samples, making it ideal for use in Western blotting and immunofluorescence applications.VPS16 is a crucial component of the multimeric CORVET complex, which is involved in the tethering and fusion of endosomes to promote protein sorting and recycling.
Dysregulation of VPS16 function has been linked to various diseases, including neurodegenerative disorders and cancer. By targeting VPS16 with this antibody, researchers can gain insights into its role in cellular processes and disease pathogenesis.With its high reactivity and specificity, the VPS16 Polyclonal Antibody is a valuable tool for studying VPS16 in various cell types and experimental settings, making it an essential reagent for researchers in the fields of cell biology, molecular biology, and biomedicine.
Product Name:
VPS16 Rabbit Polyclonal Antibody
SKU:
CAB15888
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 540-839 of human VPS16 (NP_072097.2).
Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Late endosome membrane, Lysosome membrane, Peripheral membrane protein, autophagosome, clathrin-coated vesicle.
Calculated MW:
95kDa
Observed MW:
95kDa
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
64601
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS16 Rabbit pAb (CAB15888) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
