The VMA ELISA Kit is designed for the quantitative detection of Vanillylmandelic Acid (VMA) levels in various biological samples.
VMA is a metabolite of epinephrine and norepinephrine, and its levels can be used as a biomarker for diseases like neuroblastoma and pheochromocytoma. Accurate measurement of VMA is critical for uncovering its role in disease mechanisms, as well as aiding in diagnostic processes and the development of targeted therapeutic strategies. Assay Genie’s VMA ELISA Kit offers outstanding sensitivity and specificity, ensuring trustworthy and replicable results. Fabricated under stringent quality control standards, this kit offers robust performance, simplicity of use, and is an excellent choice for both research and clinical applications. Trust Assay Genie’s VMA ELISA Kit for accurate and reliable quantification of this important biomarker in your studies.
Product Name:
VMA (Vanillylmandelic Acid) ELISA Kit
SKU:
AEES00009
Target:
VMA (Vanillylmandelic Acid)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VMA. During the reaction, VMA in the sample or standard competes with a fixed amount of VMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
97-111
97-115
Average (%)
101
104
105
1:4
Range (%)
84-99
86-100
95-106
Average (%)
90
92
101
1:8
Range (%)
84-96
87-103
99-114
Average (%)
91
94
104
1:16
Range (%)
88-103
93-105
97-112
Average (%)
95
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
85-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.6
10.9
45.9
4.8
11.8
49.4
Standard deviation
0.3
0.5
1.5
0.2
0.5
2.3
C V (%)
6.52
4.59
3.27
4.17
4.24
4.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VMA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes VMA in samples. No significant cross-reactivity or interference between VMA and analogues was observed.
