The Vitamin D3 Receptor Polyclonal Antibody (CAB11743) is a key tool for research involving the Vitamin D3 receptor, a critical regulator of gene transcription and cell signaling in response to Vitamin D3. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot and immunohistochemistry applications. By specifically targeting the Vitamin D3 receptor protein, researchers can effectively study its function and role in various cellular processes.The Vitamin D3 receptor, also known as VDR, plays a crucial role in maintaining calcium and phosphorus homeostasis, as well as regulating immune responses, cell growth, and differentiation.
Dysregulation of the Vitamin D3 receptor has been linked to various diseases, including osteoporosis, autoimmune disorders, and certain types of cancer. Therefore, studying the activity of the Vitamin D3 receptor using this antibody is essential for advancing our understanding of these conditions and potentially developing targeted therapies.
Product Name:
VDR Rabbit Polyclonal Antibody
SKU:
CAB11743
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human VDR (NP_000367.1).
This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Purification Method:
Affinity purification
Gene ID:
7421
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using VDR antibody (CAB11743) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
