The Vinculin Rabbit Monoclonal Antibody (CAB2752) is a highly specific tool for researchers studying vinculin, a key protein involved in cell adhesion and cytoskeleton organization. This antibody, generated in rabbits, offers exceptional sensitivity and specificity when detecting vinculin in human samples, making it a reliable choice for Western blot experiments.Vinculin is a critical component in cell adhesion complexes, playing a fundamental role in maintaining cellular structure and function. Dysregulation of vinculin has been implicated in various diseases, including cancer and cardiovascular disorders, highlighting its importance in physiological processes.
By using the Vinculin Rabbit Monoclonal Antibody, researchers can explore the functions of vinculin in different cell types and investigate its potential role in disease development.Given its high reactivity and reliability in detecting vinculin protein, this antibody is an essential tool for studies in cell biology, cancer research, and other fields where understanding cell adhesion mechanisms is essential. Its versatility and specificity make it a valuable asset for investigating the molecular pathways involving vinculin and its potential as a therapeutic target in disease treatment strategies.
Product Name:
Vinculin Rabbit Monoclonal Antibody
SKU:
CAB2752
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).
Sequence:
DAKA VAGN ISDP GLQK SFLD SGYR ILGA VAKV REAF QPQE PDFP PPPP DLEQ LRLT DELA PPKP PLPE GEVP PPRP PPPE EKDE EFPE QKAG EVIN QPMM M
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
7414
Clone Number:
ARC51900
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Vinculin Rabbit mAb (CAB2752) at 1:250000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
