The Vinculin Polyclonal Antibody (CAB1758) is a valuable tool for researchers studying vinculin, a key protein involved in cell adhesion and cytoskeleton organization. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Vinculin is essential for various cellular processes, including cell migration, cell shape regulation, and signal transduction. By targeting the vinculin protein, this antibody enables researchers to detect and analyze vinculin expression in different cell types, providing critical insights into cell adhesion mechanisms and potential therapeutic targets.
With its ability to specifically bind to vinculin, the CAB1758 antibody is ideal for investigations in cell biology, cancer research, and developmental biology. Understanding the role of vinculin in cell adhesion and signaling pathways is crucial for unraveling the complexities of cellular function and disease processes. Researchers can leverage this antibody to further their studies on vinculin and its implications in health and disease.
Product Name:
Vinculin Rabbit Polyclonal Antibody
SKU:
CAB1758
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 767-1066 of human Vinculin (NP_003364.1).
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
7414
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Vinculin antibody (CAB1758) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
