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Vimentin Polyclonal Antibody (CAB0326)

SKU:
CAB0326
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
€129
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Vimentin Polyclonal Antibody
SKU:CAB0326
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 10-101 of human Vimentin (NP_003371.2).
Sequence:SYRR MFGG PGTA SRPS SSRS YVTT STRT YSLG SALR PSTS RSLY ASSP GGVY ATRS SAVR LRSS VPGV RLLQ DSVD FSLA DAIN TEFK NTRT
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:CTRCT30; HEL113; Vimentin; VIM; vimentin
Positive Sample:Jurkat,Raji,Mouse eye,Rat eye
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:54kDa
Observed MW:56kDa

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Purification Method:Affinity purification
Gene ID:7431
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Vimentin Rabbit pAb (CAB0326) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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