The Villin-1 Polyclonal Antibody (CAB5494) is a vital tool for researchers studying villin-1, a key protein involved in the organization and maintenance of the actin cytoskeleton in epithelial cells. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the villin-1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an invaluable asset for studies in cell biology, cytoskeletal dynamics, and cancer research.Villin-1 is essential for the proper functioning of epithelial cells, playing a crucial role in cell shape, movement, and adhesion.
Dysregulation of villin-1 has been implicated in various diseases, including cancer, inflammatory bowel disease, and kidney disorders. By investigating the role of villin-1 in these conditions, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets. The Villin-1 Polyclonal Antibody provides a reliable tool for dissecting the functions of this important protein, driving advancements in our understanding of cellular processes and disease pathology.
Product Name:
Villin1 Rabbit Polyclonal Antibody
SKU:
CAB5494
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-827 of human Villin1 (NP_009058.2).
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.
Purification Method:
Affinity purification
Gene ID:
7429
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Villin1 antibody (CAB5494) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
